摘要
目的：建立同时测定新疆紫草中紫草酸、紫草素、乙酰紫草素、去氧紫草素、异丁酰紫草素、β,β-二甲基丙烯酰紫草素、异戊酰紫草素7种成分的HPLC检测方法。方法：选用安捷伦ZORBAX SB-C18（4.6 mm×250 mm,5μm）色谱柱,以乙腈-0.03%甲酸为流动相进行梯度洗脱,检测波长516,254 nm,柱温30℃,进样量6μL,流速1 mL·min-1。结果：7种萘醌类对照品在进样量范围内分别与峰面积呈现的良好线性关系（r〉0.999 1）;仪器精密度和稳定性RSD均〈3%;加样回收率在97%~103%（n=3）。结论：该方法快速、准确、重复性好,可同时定量新疆紫草的7种水溶性及脂溶性萘醌类成分,为新疆紫草的质量评价提供了更为全面的科学依据。
Objective： To establish a new RP-HPLC method for simultaneous determination of the seven naphthoquinones in crude extract of Arnebia euchroma,namely lithospermum acid,shikonin,acetyl shikonin,deoxyshikonin,isobutyryl shikonin,β,β-dimethylacry shikonin and isovalerylshikonin.Method： Agilent ZORBAX SB-C 18（4.6 mm ×250 mm,5 μm） column was used with acetonitrile-pure water（with 0.03% formic acid） as mobile phase in gradients elution.The flow rate was 1 mL·min-1,and the UV detector was monitored at 254,516 nm.Result： All the calibration curves of the seven naphthoquinones showed good linearity in test ranges（r 0.999 1）;and the overall recoveries were in the range of 97%-103%（n =3） with RSD less than 3%.The overall of RSD of precision test were less than 3%.A HPLC method for simultaneous determination of the seven naphthoquinones in crude extract of A.euchroma was established.Conclusion： The established HPLC method is rapid,accurate,credible and repeatable for the determination of the seven water-soluble（lithospermum acid） and liposoluble（the rest of six components） components simultaneously,and provide a more comprehensive scientific basis for the quality evaluation of A.euchroma.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第18期108-112,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(61173098)
