摘要
用自制的异硫氰酸异鲁米诺标记 AFP抗体 ,标记率平均为 0 .40 ;标记的抗体免疫活性和电致化学发光效率基本不变 ;研究了标记反应、双抗夹心免疫反应和电致化学发光反应的条件 ,建立了一种测定甲胎蛋白 ( α fetoprotein,简称 AFP)的双抗夹心电致化学发光免疫分析新方法 ,并用于测定人血清中的 AFP,方法线性范围为 5.0～ 1 0 0 .0 ng m L,检测限为 2 .0 ng m L,本方法与放射免疫法比较 ,相关性良好 .
A new chemiluminescent reagent isothiocyanato\|isoluminol (ITCI) was used to label Anti\|AFP. The molar incorporation ratios are estimated to be 0.25～0.50 mole of ITCI per mole Anti\|AFP. The results show that there are no apparent changes in the immunoreactivity of the labeled Anti\|AFP and the quantum efficiency of ITCI attached to the Anti\|AFP. In addition, the conditions of labeling reaction,sandwich immunoassay and electrochemiluminescent reaction were studied. Morever, a new sandwich electrochemiluminescence immunoassay(ECLIA) is firstly established, and used to detect AFP in human serum. The calibration ranges is 5.0～100.0 ng/mL and the minimal detection concentration is 2.0 ng/mL.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1999年第8期1194-1199,共6页
Chemical Journal of Chinese Universities
基金
国家自然科学基金! (批准号 :2 9675 0 0 3)
国家教育部材料和生命过程分析科学开放研究实验室资助
关键词
电致化学发光
免疫分析
ECLIA
甲胎蛋白
AFP
Electrochemiluminescence Immunoassay(ECLIA), Isothiocyanatoisoluminol, \{\%α\%\|Fetoprotein\}, Label
