摘要
目的建立人促甲状腺激素 (TSH)的免疫 PCR方法。找到一种高特异性高灵敏度的简便快速的方法检测低浓度抗原分子。方法用单克隆抗体包被 Eppendorf管 ,生物素化多抗和游离的亲和素作为连接分子 ,生物素化重组质粒作为指示分子 ,用特异引物扩增指示分子 ,同时与放射免疫技术、免疫放射技术和 EL ISA方法作比较。结果这种改进的免疫 PCR技术可检测到 1× 10 - 1 8mol/ L TSH含量比常规与法检测下限增高了 10 5 倍。结论这种方法的应用也为临床大量样品的检测提供了可能。
ObjectiveThe double antibodies sandwich immuno PCR(IM PCR) was constructed for detecting TSH. A high sensitivity method to investigate Ag existed in low concentration was found. MethodsCoated eppenbdorf with monoclone antibodies, used bio polyclone antibodies to TSH and free avidin as linking molecules, bio DNA as mark molecule, and amplified mark molecule with special primers. Finally compared modified IM PCR to RIA,IRMA and ELISA methods at the same time. ResultsUsed the IM PCR,nearly 1×10 -18 mol/L TSH can be investiged which is more sensitive 10 5 times than routine methods.More sensitive 10 5 times than routine methods.Conclusion Our IM PCR also supplied possibility for detecting lots of samples in clinical assay. [
出处
《免疫学杂志》
CAS
CSCD
北大核心
2000年第2期142-145,共4页
Immunological Journal
