摘要
研究了6-[N-(4-氨基丁基-N-乙基)-N-乙基]-氨基-2,3-二氢吩噻嗪1,4-二酮(ABEI)的电致化学发光的各种条件,发现最佳的电脉冲参数为:占空比0.45,脉冲周期20ms,脉冲幅值+1.6V(相对饱和甘汞电极);最佳介质是0.12mol/L KOH-0.080mol/L H_3BO_(3-)0.080 mol/L KCI(pH12.0).在这些条件下,ABEI的发光强度与浓度在1.0×10^(-8)～9.0×10^(-5)mol/L范围内呈线性关系.研究表明,溶液中的Cl^-先被氧化为CIO^-,CIO^-再与ABEI作用并使其发光.
6- [N- ( 4-aminobutyl-N-ethyl) -N-ethyl]-amino- 2,3-dihydrophthalazine 1,4-dione (ABEI) is a kind of common labelled compounds in luminescence immunodiagnosis. We have made a study of the various conditions of electrochemiluminescence of ABEI and found that the optimal rectangular electric pulse parameters are: pulse space 0. 45, pulse length 20 ms, pulse amptitude +1. 6 V(vs. SCE), and that the medium condition is: 0. 12 mol/L KOH-0. 080 mol/L H3BO3-0. 080 mol/LKCl (pH 12. 0). Under these conditions, the range of the linear relationship between light intensity and concentration of ABEI is 1.0×10-8 mol/L to 9. 0×10-5 mol/L. Our study also shows that Cl- in the solution was oxidized to ClO-, and ClO- acts on ABEI and then gives out luminescence.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1989年第11期1110-1113,共4页
Chemical Journal of Chinese Universities
关键词
ABSE
电致化学发光
免疫发光分析
Electrochemiluminescence, ABEI, Luminescence immunodiagnosis
