摘要
目的探讨香菇多糖(LNT)改善衰老小鼠学习记忆能力的抗氧化机制。方法建立D-半乳糖(D-gal)致小鼠学习记忆障碍模型,跳台实验检测其学习记忆能力,检测大脑超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量和谷胱甘肽过氧化物酶(GSH-Px)活性,观察小鼠灌胃LNT后上述指标变化。结果与正常对照组小鼠相比,D-gal模型组小鼠错误次数明显增加,潜伏期明显缩短,学习记忆能力明显下降。LNT在30和60 mg.kg-1.d-1两个剂量时,可使小鼠脑组织中SOD和GSH-Px活性明显提高,与小鼠表现的错误次数成负相关,而与潜伏期成正相关;同时可使脑组织中MDA含量明显降低,与小鼠表现的错误次数成正相关,而与潜伏期成负相关。结论 LNT可改善D-gal致学习记忆障碍小鼠的学习记忆能力,其机制可能与其抗氧化作用有关。
Objective To investigate the antioxidant mechanisms underlying the improving effects of lentinan （LNT） on learning and memory function in D-galaetose （D-gal） inducing aging mice. Methods The aging mice were induced by subcutaneous injection of Dgal for 7 weeks. The learning and memory function of mice was tested by step down test. Not only the contents of malonaldehyde （MDA） but also the activities of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） in brain tissues were determined using different biochemical methods. Results The learning and memory dysfunction, with lowered SOD and GSH-Px activities and increased MDA contents, which was found in D-gal induced aging mice as compared with the control group. LNT, at the dose of 30 and 60 mg · kg^-1 · d^-1 markedly elevated SOD and GSH-Px activities in the brain of mice treated with D-gal, showing a strong negative correlation with number of errors and a strong positive correlation with latency. Meanwhile, LNT at the dose of 30 and 60 mg · kg^-1 . d^-1 also lowered MDA contents in the brain of mice treated with D-gal, exhibiting a strong positive correlation with number of errors and a strong negative correlation with latency. Conclusions LNT can improve learning and memory function in D-gal inducing aging mice, which may be due to its antioxidant activity.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2011年第4期613-615,共3页
Chinese Journal of Gerontology
基金
山东省自然基金课题(No.2007C063)
山东省教育厅科研课题(No.J07YD11)
关键词
香菇多糖
D-半乳糖
学习记忆
抗氧化
Lentinan （LNT）
D- galaetose （D-gal）
Learning and memory
Antioxidation
