摘要
为了进一步证实P6-9紫茎性状与可育性状的连锁关系,本试验选用S45A×自1968的BC_1F_1群体,利用BSA分析法,筛选到两个与可育基因Ms_1连锁的RAPD片段:UBC_(158.580)和UBC_(187.880).通过对可育株群体的分析,证明了P6-9紫茎基因Pur与Ms_1基因以及UBC_(158.580)和UBC_(187.880)位于同一连锁群上;对不育株分析得出其在同一连锁群上的精确位置,Pur(3.25cM)—Ms_1(8.67cM)—UBC_(158.580)(8.78.M)—UBL_(18,880).
In order to identify the linkage relationship between P6-9 purple stem gene and the male fertility gene, the BC1F1 population from S45AxxxxxxxxxxxZi 1968 was investigated by BSA (Bulked Segregant Analysis) and found out two RAPD fragments, UBC158.580 and UBC187.880, linked to the purple stem gene and the male fertile gene(Ms1). The results show that these four genes are located the same linkage group, and the order is as follow: Pur(3. 25 cm)-Ms1(8. 67 cm)-UBC158.580(8.78cm)-UBC187.880.
出处
《作物学报》
CAS
CSCD
北大核心
1999年第6期669-673,共5页
Acta Agronomica Sinica
基金
国家攀登计划"粮棉
油雄性不育杂种优势基础研究资助"项目
