摘要
以籼稻栽培品种秋桂矮１１的愈伤组织为材料，对铜元素的作用作了进一步的研究。在含有２，４-Ｄ和脯氨酸的培养基中添加银元素对愈伤组织的增殖量影响极小，但转移到再分化培养基后这些愈伤组织能再生出显著更多的植株。另一方面，在含有ＢＡ、ＮＡＡ和脯氨酸的培养基中添加钢元素则会由于添加量的不同而产生显著的促进或抑制愈伤组织增殖的作用。然而，不论是增殖受过铜的促进的或者是抑制的愈伤组织，均能在再分化培养基上再生出数量更多和质量更好的植株。
Calli of an indica rice cultivar Qiuguiai 11 were used as material for a further study on the effects of copper in calli cultures. Supplement of copper to a medium containing 2,4-D and proline had little effect on the proliferation rate of the calli,however, upon transfer to the plant regeneration medium, these calli would regenerate significantly more number of plants. Onthe other hand, supplement of copper to a medium containing proline, BA and NAA would significantly stimulate or suppressthe proliferation of the calli depending on the amount of copper supplemented. Nevertheless, all these calli, no matter the proliferation of which had been stimulated or suppressed by the copper, were able to regenerate more number of plants with higherquality on plant regeneration medium.
出处
《中国水稻科学》
CAS
CSCD
北大核心
1999年第4期245-247,共3页
Chinese Journal of Rice Science
关键词
组织培养
增殖
植株再生
铜元素
水稻
callus culture
proliferation
plant regeneration
copper
rice
