摘要
研究了植物生长延缓剂多效唑在组织培养中对水稻愈伤组织诱导、分化以及壮苗培养的影响。结果表明:(1)多效唑(MET)能抑制未成熟胚愈伤组织的诱导率而能明显地提高愈伤组织的绿苗形成率,以MET 2.5 mg/1时效果较好:(2)MET明显地抑制再生苗地上部生长而促进根系的发育,这种抑制效果通常保持2个月左右,不过MET对叶龄的影响不大,(3)MET处理的再生苗移栽后成活率为95%左右而且生长健壮;(4)与对照相比,MET处理的再生苗具有较大的维管束,较厚的叶肉细胞、致密的表皮细胞,并存维管束细胞中有较多的营养物质。
The results indicated that: (1) Multi-Effect Triazole (MET) inhibitcd the callus induction from immature embryo. However, the callus redifferentiation and green plantlet formation were promoted significantly, especially with lower concentration of MET (2.5mg/l); (2) MET inhibited the plant height obviously and promoted the development of the root system of plantlet, such effect usually maintained for about 2 months But the effect of MET on leafage was not significant; (3) The survival rate of MET hardened plantlets was about 95% and the plantlcts grew more vigorously after transplanting; (4) compared with the control, the MET treated plantlets contained bigger vascular, thicker mesophyll cells, denser surface cells and more inclusion in the vascular cells.
出处
《中国水稻科学》
CAS
CSCD
北大核心
1990年第4期169-174,共6页
Chinese Journal of Rice Science
关键词
水稻
试管苗
多效唑
离体调控
Multi-Effect Triazole
Immature embryo
Callus induction
Plant tissue culture
Rice
