摘要
目的观察移植于脑内的骨髓基质干细胞(BMSCs)对创伤脑组织的神经修复作用。方法流式细胞术鉴定原代培养的大鼠BMSCs,免疫荧光技术检测5-溴脱氧尿苷(BrdU)标记BMSCs的比例;建立大鼠颅脑创伤模型后在创伤灶周边进行BMSCs移植,免疫组织化学方法检测BMSCs在脑创伤灶内的分布和神经分化情况;实验动物予以神经功能评分。结果流式细胞术检测结果符合大鼠BMSCs特征,体外BrdU标记的BMSCs呈现强的红色胞核荧光;体内移植显示BMSCs在脑内成功存活,主要分布于创伤灶内或其边缘,并部分表达神经标志蛋白;BMSCs移植组实验动物神经功能评分明显优于对照组。结论脑内移植的BMSCs经过短距离迁移定位于创伤灶区域,并通过向神经细胞方向的分化实现部分神经修复和功能代偿。
Objective To observe the neural repair of traumatic brain injury by the transplanted bone marrow stromal stem cells（BMSCs）.Methods BMSCs were identified by flow cytometry analysis and the ratio of BMSCs labeled by 5-bromodeoxy-uridine（BrdU）was detected by the immunfluorescence assay.BMSCs were injected into the traumatic brain cortex of rats,and the samples were prepared for immunohistochemistry to detect the distribution and differentiation of the transplanted BMSCs.Neurological function scores were recorded.ResultsFlow cytometry assay confirmed that BMSCs were obtained successfully,and the BMSCs labeled by BrdU showed strong red fluorescence in neuclei.Transplantation in vivo showed that survived BMSCs mainly located in or around traumatic foci with expression of special neural proteins in certain degree.Neurological function score of animals in group with BMSCs transplantation was obvious higher than that of control group.Conclusion The transplanted BMSCs could distribute in the traumatic foci of rat brain and differentiate into neural cells for the neural repair and functional recovery.
出处
《中华神经外科疾病研究杂志》
CAS
2011年第1期8-11,共4页
Chinese Journal of Neurosurgical Disease Research
关键词
干细胞
移植
分化
Stem cells
Transplantation
Differentiation
