摘要
目的了解局灶性脑缺血再灌注大鼠脑组织中Nestin的表达特点。方法采用改良的线栓法制备大脑中动脉阻塞(MCAO)2h后不同再灌注时间段(6h、12h、24h、72h、7d、14d)的大鼠局灶性脑缺血模型,应用免疫组织化学技术检测Nestin的表达。结果脑缺血再灌注后6h,Nestin+表达即有增加,至3d时Nestin+表达显著增强,Brdu+/Nestin+细胞也已明显可见,至7d时Nestin+表达、Brdu+/Nestin+细胞数目均达顶峰,至再灌注14dNestin+表达及Brdu+/Nestin+细胞数目均已明显回落。结论脑缺血可引发大鼠Nestin的表达上调,并可诱导神经干细胞增殖。
Objective To study the expession of Nestin after focal ischemia-reperfusion in rats. Methods The model of acute reperfusion injury after cerebral iscbemia in rat was made by middle cerebral artery occlusion. All animals were allowed 2 h iscbemia and sacrificed after different durations of reperfusion （6 h, 12 h, 24 h, 72 h, 7d, 14 d）. The expression of Nestin was detected with an immunobistocbemical approach. Results The number of Nestin-immunoreactive cells at 6 h after reperfusion in the iscbemic side started to increase markedly and showed significant difference, compared with the Sham group （P〈0. 05）. The expression of Nestin and the number of Nestin positive cells with Brdu labeled peaked at 7 d after reperfusion and then were down-regulated at 14 d. Conclusion The study implies cerebral iscbemia could upregulate the expression of Nestin and induce the proliferation of nervous stem cells.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2005年第5期645-648,共4页
Journal of Sichuan University(Medical Sciences)
