摘要
目的探讨青霉素全身给药建立的大鼠癫痫模型在癫痫发作不同时间不同脑区的T2弛豫时间的改变。材料与方法成年健康雄性SD大鼠10只,腹腔注射青霉素300万U/kg体重×7天,分别于青霉素注射前、注射结束当日及注射后7天行MR成像,并对顶叶运动皮层、颞叶皮层、梨状皮层、齿状回、海马及侧脑室区域进行T2弛豫时间测量。结果青霉素全身给药建立的癫痫模型大鼠的行为表现以强直和阵挛为主。青霉素注射结束当日及注射后7天部分模型大鼠侧脑室区域在T2WI上出现异常信号;T2弛豫时间测量结果显示,青霉素注射结束当日及注射后7天双侧海马CA1区和侧脑室区域的T2弛豫时间没有明显差异,但相对于注射前有明显的差异(P<0.05)。T2WI检测侧脑室区域信号增强,组织学检测证实为侧脑室扩大。结论癫痫模型中脑局部区域出现T2弛豫时间明显变化,这些区域可能是导致癫痫发作或与癫痫发作有关的重要神经生物区域。
Objective To investigate the difference of T2 relaxation time in different brain regions of penicillin-inducedepilepsy rats. Materials and Methods 10 male Sprague Dawley rats were injected intraperitoneally with penicillin （600 000 U/kg ×7 ） , and then MR imaging was underwent at different time. T2 relaxation time of the motor Cortex of parietal lobe, temporal lobe, dentate gyrus, hippocampus and lateral ventricle region was measured with muhieeho spin echo sequences. Results The rat models induced by penicillin were showed tonus and clonus in the present study. Hippoeampus and lateral ventricle region had significant signal abnormalites in T2 weighted magnetic resonance image （T2WI）. Quantification of the T2 relaxation time was demonstrated a significant increase in hippocampus and lateral ventricle region of postpenicillin-induced-epilepsy 7 days after than of pre-penicillin-indueed-epilepsy rat brain. T2WI hyperintensity in lateral ventricle region was resulted by enlargement of lateral ventricle. Conclusion The results of this study validate a method and demonstrate the induction of T2 signal abnormality in focal regions of the hippocampus and lateral ventricle. These regions may be important sites for the neurobiologic changes that contribute to the epileptogenesis in this model.
出处
《临床放射学杂志》
CSCD
北大核心
2009年第4期574-577,共4页
Journal of Clinical Radiology
基金
国家基础研究项目973资助(编号:2006,CB705707)
关键词
癫痫
青霉素
T2弛豫时间
Epilepsy Penicillin T2 relaxation time
