摘要
目的研究成年大鼠脑出血模型中发生的神经细胞再生现象及其特点。方法应用雄性Sprague—Dawley大鼠制作脑出血模型，采用蛋白印迹分析、免疫组化染色等方法对5-溴脱氧尿嘧啶核苷（Bromodeoxyuridine，Brdu）及未成熟神经元标记doublecortin（DCX）等进行分析。结果DCX的表达于脑出血后7d开始增加，2周后同侧脑室下区（SVZ）及基底节区可见大量DCX阳性细胞，其表达达到高峰，为对照组的（793±192）％（P〈0．01）。结论成年大鼠中SVZ的神经元前体细胞在脑出血后明显增生，并向受损伤区域移行。
Objective To investigate neurogenesis in adult rat after intracerebral hemorrhage. Method Male Sprague-Dawley rats received an intracerebral infusion of 100μl autologous blood. Western blot analysis and histological examination were performed to check doublecortin （ DCX ） and Bromodeoxyuridine（Brdu）. Results DCX levels in the ipsilateral basal ganglia started to increase as early as seven days after intracerebral hemorrhage （ ICH）, peaked at 14 days （793±192 % of sham, P 〈 0.01 ）. We found that most DCX positive cells were Brdu positive. Conclusion The results demonstrated that neurogenesis occurs from subventricular zone（SVZ） in the brain following ICH.
出处
《中华神经外科杂志》
CSCD
北大核心
2008年第9期710-712,共3页
Chinese Journal of Neurosurgery
基金
浙江省自然科学基金资助项目（Y205055）
