摘要
实验采用VP-ODS色谱柱(150 mm×4.6 mm,5.0μm),以5%甲醇-10%乙腈-85%30 mM磷酸缓冲液(pH3.0,体积比)为流动相;流速为1.0 mL/min,检测波长为285 nm.该方法对13种喹诺酮类药物的线性范围为0.03～100μg/mL,最低检测限为0.001～0.03μg/mL;样品萃取回收率均大于64%,方法简单,准确,为食品中喹诺酮类药物的残留检测提供了一种有效的分析方法.
The good chromatographic separation was achieved on a VP-ODS column（150mm×4.6mm,5.0μm） through variation of the mobile phase additive combinations,which consisted of 5% methanol,10% acetonitrile and 85% 30mM phosphate buffer solution（pH 3.0） at a flow rate of 1.0mL/min under an optimization isometric program,allowed to accomplish the chromatographic separation in 46min.UV detector wavelength was set at 285nm.The method was linear over the concentration range of 0.03 ～ 30.0μg/mL or 0.10 ～ 100μg/mL for thirteen（fluoro）quinolones,and the detection limits were between 0.001 ～ 0.03μg/mL.The solid-phase extraction recovery of the thirteen（fluoro）quinolones from honey sample were beyond 64%.This method will provide the method for the multiresidue determination of drugs in food.
出处
《赣南师范学院学报》
2010年第6期61-66,共6页
Journal of Gannan Teachers' College(Social Science(2))
关键词
反相高效液相色谱
喹诺酮
蜂蜜
reversed-phase high-performance liquid chromatography
quinolones
honey
