摘要
采用高效液相色谱-质谱联用法测定豆芽中恩诺沙星的含量。样品经0.1mol/L NaOH溶液提取后,经WatersOasis MAX阴离子交换柱净化,以双氟沙星为内标,采用反相高效液相色谱分离,以乙腈-水(含0.1%甲酸)为流动相进行梯度洗脱,通过带有电喷雾离子化源的串联质谱仪,应用正离子扫描,在多反应监测模式下进行定性与定量分析。本法线性范围为1～100μg/kg,平均回收率为91.6%～94.0%,相对标准偏差不大于6.6%,方法的检出限为0.3μg/kg。
This paper reports a sensitive and specific LC-MS/MS method for the determination of enrofloxacin in soy bean sprout.Samples were extracted with 0.1 mol/L NaOH,cleaned up on a Waters Oasis MAX anion exchange column,and separated by RP HPLC using a mobile phase consisting of acetonitrile and 0.1% formic acid for gradient elution.Enrofloxacin was quantitatively and qualitatively analyzed by ESI-MS/MS in the positive ion scan MRM mode.The method showed a linear range of 1–100 μg/kg and a limit of detection of 0.3 μg/kg.The average spike recovery rates of enrofloxacin in blank sample was 91.6%–94.0% with RSD of 6.6%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第20期180-183,共4页
Food Science
关键词
高效液相色谱-质谱联用
恩诺沙星
双氟沙星
豆芽
high performance liquid chromatography-tandem mass spectrometry（LC-MS/MS）
enrofloxacin
difloxacin
soy bean sprout
