摘要
报道了一种高效快速检测抗癌药物巯嘌呤的方法——毛细管区带电泳柱端安培检测.考察了巯嘌呤的电化学行为.在最佳操作条件下,获得此药物的检测限为1×10^(-7)mol/L;线性范围为5×10^(-4)～5×10^(-6)mol/L;相关系数为0.995,重现性良好.并用该法检测了人尿样及牛血清蛋白中的巯嘌呤.
End-column amperometric detecdon of 6-mercaptopurine by capillary zone electrophoresis was described with high resolution and speed. Hie detecdon condidons were optimized and the electrochemical behavior was observed. Under the optimal condidons: detection potential of 1.2 V (vs. Ag/AgCl), operating voltage of 15 kV, sample injection of 3 s at 15 kV and 10 mmol/L Na2HPO4 buffer,the detection limit for 6-MP was as low as 1×10-7 mol/L and the linear range was from 5×10-4 to 5×10-6 mol/L with the relative coefficient of 0. 995. The BSD of reproducibility for peak current andmigration time was 2.5% and 1.2%, respectively. This method was utilized in assay real sample ofhuman urine and bovine serum albumin containing 6-mercaptopurine.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1999年第10期1141-1145,共5页
Chinese Journal of Analytical Chemistry
关键词
毛细管电泳
安培检测
巯嘌呤
测定
抗癌药
Capillary electrophoresis, amperometric detection, 6-mercaptopurine
