摘要
目的：观察Ｖｅｒａｐａｍｉｌ（异搏定）对体外培养角膜基质细胞的影响，为其临床应用提供基础。方法：进行兔角膜基质细胞的原代和早期传代培养，并用ＭＴＴ自动比色法检测Ｖｅｒａｐａｍｉｌ对兔角膜基质细胞增殖的影响。结果：Ｖｅｒａｐａｍｉｌ浓度１０～１０００μｇ／ｍｌ作用４８ｈ和７２ｈ对角膜基质细胞增殖有明显抑制作用（Ｐ＜０．０５）。作用７２ｈ抑制率高于作用４８ｈ。结论：Ｖｅｒａｐａｍｉｌ为剂量依赖型和时间依赖型药物，能有效地抑制角膜基质细胞增殖，可望成为调节角膜伤口愈合的新药物。
Objective:To observe the effect of Verapamil on keratocytes proliferation in vitro,and provide basic experiment for its clinical application.Methods:The rabbitskeratocytes were cultured in vitro,and the earlypassage cells were used for this study.The effect of Verapamil on the proliferation of rabbits keratocytes were examined by MTT automated colorimetry.Results:Verapamil (10～1000μg/ml) could inhibit the proliferation of keratocytes after incubation for 48h and 72h (P<0 05).Inhibition rates after 72h were higher than those after 24h.Conclusion:Verapamil was dose dependent and time dependent inhibitors.Verapamil might become a new drug for regulation of corneal wound healing.
出处
《中国实用眼科杂志》
CSCD
1999年第7期399-401,共3页
Chinese Journal of Practical Ophthalmology
