摘要
目的建立从同一角膜片上分离培养角膜上皮、基质和内皮细胞的方法。方法采用消化法培养猴角膜内皮细胞、上皮细胞和兔角膜上皮细胞，组织块培养法培养角膜基质细胞和兔角膜内皮细胞。结果猴、兔角膜内皮细胞培养１周后，均能形成细胞单层；猴角膜上皮细胞培养３～４天生长旺盛，但难以形成细胞单层，兔上皮细胞生长旺盛，１周后已达融合状态，细胞呈膜状伸出板层伪足；猴、兔基质细胞易培养，１周后融合成单层细胞。结论从同一角膜中能分别培养出角膜３种细胞，消化法和组织块培养法相结合既节省材料，又简单易行。
ObjectiveToinvestigatetheculturetechniqueforcornealepithelialcells,keratocytesandendothelialcellsfromindividualcornealmaterials.MethodsThecornealendothelialcells,epithelialcellsformonkeysandcornealepithelialcellsforrabbitswereculturedbyusingdigestionmethods.Thekeratocytesandendothelialcellsforrabbitswereculturedbytissueculturemethods.Allcellsandsmalltissueswereplantedin12wellcultureplateandthemorphologicalcharacteristicsofculturecellswereobservedbyWrightstainingatvarioustimeperiods.ResultsAtoneweekculture,theendothelialcellsformonkeysandrabbitsformedanadherentandconfluentmonolayer,andthehexagoncellswassimilarthatinvivo.Theepithelialcellsformonkeysgrewverywellat3 or4dculture.Theepithelialcellsforrabbitscanformaconfluentmonolayerandthecellspossessedaflattenedlamellipodia.Thekeratocytesformonkeysandrabbitsformedamonolayerandhadafibriformarrangementafteroneweekculture.ConclusionThreekindsofcellsfromthesamecorneacanbeculturedsuccessfully.Theculturetechniqueispracticableandsimple.
出处
《眼科研究》
CSCD
1999年第3期191-194,共4页
Chinese Ophthalmic Research
基金
上海市科学技术发展基金
关键词
角膜细胞培养
上皮细胞
基质细胞
内皮细胞
corneacellcultureepitheliumkeratocyteendothelialcellmonkeyrabbit
