摘要
目的探讨上皮钙黏素（E—cad）、CD44v6和血管内皮生长因子C（VEGF—C）在食管癌组织中的表达及其与淋巴结转移的关系。方法应用免疫组织化学EnVision两步法，检测56例食管鳞状细胞癌（鳞癌）组织（区域淋巴结转移组32例，非转移组24例）和12例食管切缘正常黏膜组织中E-cad、CD44v6和VEGF-C的表达情况。结果E-cad在56例食管鳞癌组织中的阳性表达率为55．4％（31／56），显著低于食管切缘正常黏膜组织的100．0％（12／12）（P〈0．05）；CD44v6和VEGF—C在食管鳞癌组织中的阳性表达率分别为71．4％（40／56）和62．5％（35／56），显著高于食管切缘正常黏膜组织的25．0％（3／12）和16．7％（2／12）（P〈0．05）；E—cad在区域淋巴结转移组阳性表达率为31．3％（10／32），明显低于非转移组的87．5％（21／24）（P〈0．05）；CD44v6和VEGF—C在区域淋巴结转移组阳性表达率分别为84．4％（27／32）和75．0％（24／32）明显高于非转移组54．2％（13／24）和45．8％（11／24）（P〈O．05）；E—cad表达与食管癌淋巴结转移呈负相关，cD。和VEGF—C表达与淋巴结转移呈正相关，差异有统计学意义（P〈0．05）。结论E—cad、CD44v6、VEGF—C在食管鳞癌淋巴结转移过程中起重要作用，联合检测有助于判断食管癌的生物学行为和预后。
Objective To investigate the expression of E-cadherin （E-cad）, CD44v6 and VEGF-C in esophageal carcinoma and investigate their relationship with lymph node metastasis. Methods The expression of E-cad, CD44v6 and VEGF-C was detected with immunohistochemical EnVision method in 56 cases of esophageal carcinoma and 12 cases of normal esophageal mucosa. Results Among 56 cases of esophageal carcinoma, the positive expression rates of E-cad in esophageal carcinoma was 55.4 %（31/56）, significantly lower than that in normal esophageal mucosa 100.0 %（12/12） （P 〈0.05）. The positive expression rates of CD44v6 and VEGF-C were 71.4 % （40/56） and 62.5 % （35/56）, significantly higher than that in normal esophageal mucosa （P 〈0.05）. The positive rates of E-cad was 31.3 % （10/32） in the group of positive lymph - node metastasis, significantly lower than that in the group of positive non-lymph-node metastasis 87.5 % （21/ 24） （P 〈0.05）. The positive rates of CD44v6 and VEGF-C were 84.4 % （27/32） and 75.0 % （24/32） in the group of positive lymph node metastasis ,significantly higher than that in the group of positive non-lymph-node metastasis 54.2 % （13/24） and 45.8 %（11/24） （P〈0.05）. E-cad was negatively correlated with lymph node metastasis; CD~ and VEGF-C were positively correlated with lymph node metastasis （P 〈0.05）. Conclusion E-cad,CD44v6 and VEGF-C play important roles in the process of soakage and metastasis of esophageal carcinoma. The combined detection of E-cad, CD44v6 and VEGF-C can help to prodict biological behaviors and prognosis of esophageal carcinoma.
出处
《肿瘤研究与临床》
CAS
2010年第11期755-757,760,共4页
Cancer Research and Clinic
