摘要
目的探讨左归丸对去卵巢所致骨质疏松大鼠的防治作用。方法 手术切除大鼠双侧卵巢建立绝经后骨质疏松大鼠模型,用左归丸(高、中、低剂量)进行灌胃,120 d后,取大鼠左后肢股骨远端1/3做病理切片,光镜下观察骨组织形态学变化,测定骨小梁面积百分比(%Tb.Ar)、骨小梁厚度(Tb.Th)、骨小梁分离度(Tb.Sp);采用双能X线骨密度仪测定右后肢离体股骨近端1/3骨密度(BMD);采用酶联免疫吸附法(ELISA)检测大鼠血清骨钙素(BGP),用比色法测定血清抗酒石酸酸性磷酸酶(TRAP)。结果切除大鼠双侧卵巢后,骨小梁明显紊乱,变细且较稀疏,缺少连接,断端增多,%Tb.Ar、Tb.Th显著降低,Tb.Sp明显升高,BMD下降,血清BGP及TRAP水平显著升高,统计学显示有显著性差异(P<0.01);去卵巢大鼠灌服左归丸后,与模型空白组比较,左归丸各剂量组均能不同程度地改善骨小梁变化,增加BMD,降低血清BGP及TRAP,统计学显示有显著性差异(P<0.01),各用药组之间比较无显著性差异(P>0.05)。结论 左归丸对去卵巢所致绝经后骨质疏松大鼠有一定的防治作用。
Objective To explore the preventive and therapeutic effect of Zuogui Pill on ovariectomyinduced postmenopausal osteoporosis （PMOP） in rats. Methods Both ovaries were removed to establish the rat PMOP model. Zuogui Pill （high, middle and low dose） was administered through gastric tube for 120 days. Left hind limb was taken and the distal 1/3 of the femur was sectioned. Bone morphology was observed under light microscope. Percentage of trabecular bone area （% Tb·Ar） , trabecular thickness （Tb·Th） , and trabecular separation （Tb·Sp） were determined. Bone mineral density （BMD） of right hind limb at the 1/3 of proximal femurs was measured using dual X-ray absorptiometry. Serum bone Gla protein （BGP） was measured with enzyme linked immunosorbent assay （ELISA）. Serum tatrate-resistant acid phosphatase （TRAP） was measured using colourimetry. Results After removal of both ovaries, bone trabecula became disorder, thinner, relatively sparse, lack of connection, and with more fractures. % Tb·Ar and Tb·Th significantly decreased. Tb·Sp increased significantly. BMD decreased. BGP and TRAP increased significantly with statistical difference （P 〈0.01 ）. Comparing with the control model group, bone trabecula change was improved in the rats fed with Zuogui Pill. BMD increased. BGP and TRAP decreased significantly with statistical difference （ P 〈 0.01 ）. There were no significant differences among treated groups （P 〉 0.05）. Conclusion Zuogui Pill has the preventive and therapeutic effect on ovariectomized PMOP rats.
出处
《中国骨质疏松杂志》
CAS
CSCD
2010年第11期847-850,823,共5页
Chinese Journal of Osteoporosis
基金
国家自然科学基金项目(30873226)
关键词
左归丸
绝经后骨质疏松
去卵巢大鼠
Zuogui Pill
Postmenopausal osteoporosis
Ovariectomized rats
