摘要
建立了一种简单且适合于批量提取鸡蛋中的卵黄高磷蛋白的方法．先分离出其它水溶性蛋白质，再将沉淀物脱去脂肪，然后用１０％的ＮａＣｌ溶液（ｐＨ７．０）从所得的颗粒中提取卵黄高磷蛋白．每１００ｇ蛋黄提取１ｇ卵黄高磷蛋白粗制品，含氮１１．２２％，含磷７．７％，氮磷含量比值３．６４．卵黄高磷蛋白在ＳｅｐｈａｃｒｙｌＳ－２００图谱上有两个主峰，分别是α－ＰＶ和β－ＰＶ，相对分子质量为１６００００和１９００００．在聚丙烯酰胺凝胶电泳上出现相对应的两条谱带．经ＳＤＳ－聚丙烯酰胺凝胶电泳后，则出现相对分子质量从１０４～９×１０４左右的多条谱带。
A simple method suitable to prepare phosvitin from egg yolk on a pilot plant scale was developed, which consisted of the removal of watersoluble proteins, except phosvitin, the removal of lipids, the isolation of phosvitin from the resulted granules with 10% NaCl (pH7.0), and desalting by using ultrafiltration. A yield of 1g crude phosvitin per 100g egg yolk was obtained with N/P ratio of 3.64. Crude phosvitin was fractionated into mainly two components (PV and PV) by Sephacryl S200 gel filtration, with molecular weight of 160 000 Da and 190 000 Da respectively. Polyacrylamide gel electrophoresis of native phosvitin revealed the presence of similar polypeptide subunits. Phosvitin was dissociated into several bands, ranging in molecular weight from 10 000 to 90 000 daltons in the SDS polyacrylamidegel electrophorogram.
