摘要
为了快速、灵敏地检测禁用药物环丙沙星在水产品中的残留,建立了基于单克隆抗体的间接竞争酶联免疫法(competitive indirect ELISA,ci-ELISA),并比较了其与高效液相色谱法(High Performance LiquidChromatography,HPLC)在灵敏度、准确性和重现性等方法学参数上的差异。实验结果显示,经过浓缩、净化等前处理过程,ci-ELISA与HPLC的检测灵敏度分别可以达到10μg/kg和1μg/kg,回收率分别为79.28%～92.92%和86.27%～97.32%,相对标准偏差分别在3.21%～8.88%和2.66%～5.52%。前者经济、高效,适合作为初筛方法大批量筛选样品;后者灵敏度、重现性更好,适于在实验室条件下作为验证方法。
For sensitive and specifically detection of ciprofloxacin （CIP） residues in fishery products,this study is aimed at generating and developing a competitive indirect enzyme-linked immunosorbent assay （ciELISA） based on CIP-specific monoclonal antibodies （McAb） .The sensibility,accuracy and repeatability of ci-ELISA method was compared to those of High Performance Liquid Chromatography（HPLC） method.By concentration and purification process,the limit of detection （LOD） of ci-ELISA and HPLC method could reach to 10 μg /kg and 1 μg /kg respectively.we detected the recovery rates were 79.28%-92.92% and 86.27%-97.32% while relative standard deviation （RSD） varying from 3.21%-8.88% and 2.66%-5.52% for these methods.Between the two methos,ci-ELISA method was cheaper and more efficient while HPLC method was more sensitive and stable.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2010年第6期805-809,共5页
Journal of Shanghai Ocean University
基金
上海市科技兴农重点攻关项目(6660106477)
公益性行业(农业)科研专项(200803013)
公益性行业(农业)科研专项(nyhyzx07-042)
现代农业产业技术体系建设专项资金(NYCYTX-49-17)
关键词
环丙沙星
水产品
残留
间接酶联免疫法
高效液相色谱法
ciprofloxacin
fishery products
residues
competitive indirect ELISA
High Performance Liquid Chromatography
