摘要
采用碳二亚胺法将半抗原环丙沙星与卵清蛋白和牛血清白蛋白进行偶联。采用非变性凝胶电泳法和紫外分光光度法对偶联物中环丙沙星与载体蛋白的分子结合比进行分析。紫外分光光度法表明,环丙沙星与卵清蛋白和牛血清白蛋白的分子结合比分别为6∶1和13∶1。非变性凝胶电泳显示,即使只有6个环丙沙星分子与载体蛋白偶联,偶联物在凝胶中的迁移轨迹与载体蛋白和偶联剂处理的载体蛋白对照样品均有明显不同。结果表明,非变性凝胶电泳法和紫外分光光度法可用于环丙沙星与卵清蛋白和牛血清白蛋白分子结合比的定性分析和定量分析。
Hapten-carrier protein conjugates were made using ciprofloxacin （CPFX） and two carrier proteins by 1-ethyl-3- （3- dimethylaminopropyl ） carbodiimide hydrochloride （EDC） method. Ultraviolet spectrophotometry were used to demonstrated that the molecule conjugate ratio of CPFX to ovalbumin （OVA） and bovine serum albumin （BSA） are 6：1 and 13：1 respectively. Nondenaturing gel electrophoresis results revealed that the conjugate band migrates differently from that of the carrier protein alone and of the EDC-treated protein when as few as 6 molecules of CPFX are attached to the carrier protein. The results indicate that nondenaturing gel electrophoresis and ultraviolet spectrophotometry can be employed to analyze the molecule coupling ratio of CPFX to carrier proteins qualitatively and quantitatively.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2006年第2期200-203,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(30230620)~~
关键词
环丙沙星
半抗原
载体蛋白
分子结合比
非变性凝胶电泳
ciprofloxacin
hapten
carrier protein
molecule coupling ratio
nondenaturing gel electrophoresis
