摘要
建立了固相萃取-高效液相色谱(SPE-HPLC)同时测定蜂蜜中5种有机酸(L-苹果酸、马来酸、琥珀酸、柠檬酸、D-苹果酸)含量的方法。蜂蜜经制样后过Bond Elutes SAX固相萃取(SPE)小柱净化,用C18-MS-Ⅱ反相色谱柱(250mm×4.6mm,5μm)进行分离,流动相为2%偏磷酸溶液,流速为0.7mL/min,检测波长为210nm。在此条件下5种有机酸在相应的线性范围内其线性相关系数均大于0.9967;方法的回收率为86.0%～103.9%,相对标准偏差为5.7%～9.8%(n=6),检出限为0.06～9.4mg/kg。所建立的方法可用于蜂蜜样品中有机酸的测定。
A method for the simultaneous determination of 5 organic acids （L-malic acid,maleic acid,succinic acid,citric acid,D-malic acid） in honey was developed by solid-phase extraction and high performance liquid chromatography （SPE-HPLC）. The sample was purified by a Bond Elutes SAX SPE cartridge after pretreatment,then separated using a reversed-phase C18-MS-Ⅱcolumn. A 2% metaphosphoric acid solution was used as the mobile phase at a flow rate of 0.7 mL/min. The detection was performed by a diode array detector at 210 nm. Under these conditions,the linear ranges of the method for L-malic acid was 5.0-500 mg/L,for maleic acid was 0.1-500 mg/L,and for succinic acid,citric acid,D-malic acid were 18.0-500 mg/L. The correlation coefficients of 5 organic acids were more than 0.9967. The average recoveries of 5 organic acids were 86.0%-103.9% with the relative standard deviations of 5.7%-9.8% （n=6）. The detection limits ranged from 0.06 to 9.4 mg/kg. The method can be used for determining organic acids in honey samples.
出处
《色谱》
CAS
CSCD
北大核心
2010年第10期945-949,共5页
Chinese Journal of Chromatography
基金
武汉市科技局工程技术研究中心项目(No.200920137007)
华中农业大学新兴学科研究生教育发展基金(No.200922)
关键词
固相萃取
反相高效液相色谱法
有机酸
蜂蜜
solid-phase extraction （SPE）
reversed-phase high performance liquid chromatography （RP-HPLC）
organic acids
honey
