摘要
目的分析ｐ１６基因、细胞周期蛋白依赖性激酶（ＣＤＫ４）和视网膜母细胞瘤基因（Ｒｂ）在人肝细胞癌中表达的改变。方法用免疫组织化学方法对４０例肝细胞癌和癌分组织作检测。结果肝癌组织中的ｐ１６蛋白、ＣＤＫ４和Ｒｂ蛋白的异常表达分别为９４．４４％（３４／３６）、４０．００％（１６／４０）和４５．７１％（１６／３５）。ｐ１６蛋白阴性或弱阳性而ｐＲｂ呈强阳性者１３例（１３／３４，３８．２３％），两者的表达呈负相关关系二和Ｒｂ均异常者１５例（１５／３４，４４．１１％），表明Ｒｂ表达的调节除ｐ１６外，还有其他的途径。ＣＤＫ４过度表达的１４例中大多伴Ｐ１６的改变（１３／１４，９２．８５％），５０．００％能影响Ｒｂ蛋白的表达，同时伴ｐ１６和Ｒｂ异常者为４６．１５％。三者的异常表达与肝癌的恶性程度无相关关系。结论细胞调节因子Ｐ１６基因、ＣＤＫ４和Ｒｂ的表达异常，均参与肝细胞癌的发生。以ｐ１６蛋白表达的改变最为明显。
Objective To make an analysis of the expression of pl6 gene, CDK4 and retinoblastomaprotein(pRb) in human hepatocellular carcinoma(HCC). Methods Application of immunohistochelnistfy to40 cases of HCC and their adjacent liver tissues. Results The abnormal expressions of p16, CDK4 and PRbwere 94.44%(34/36), 40.00%(16/40) and 45.71%(16/35) respectively. Rb protein was strongly stained in 13/34(38.23%) cases lacking or weak staining with p16, which showed a negative relation between the expressions of Rb and p16. The abnormal expressions of both p16 and Rb were 15/34(44.11%) cases, indi-indicating that except p16, there are some other ways to roplate Rb. In 14 cases of CDK4 overexpression,most of them (92.85%) presented p16 aberration, 50.00% of them were observed with pRb abnormalexpression, 46.15% of them were along with the alterations of both p16 and pRb. There was no significant correlation between p16 or CDK4 or pRb abnormal expression and HCC malignant grade. ConclusionThe data show that the aberrant expression of p16, CDK4 and pRb may play a role in the carcinogenesiso f HCC. The chances of p16 expression is prominent.
出处
《中华肝脏病杂志》
CAS
CSCD
1999年第2期94-95,共2页
Chinese Journal of Hepatology
基金
国家自然科学基金!No.39060032
关键词
肝细胞癌
蛋白激酶
P16基因
RB蛋白
Carcinoma, hepatocellular Proteinkinase Genes, p16 Protein Rb
