摘要
探讨抑癌基因在哈萨克族（哈族）食管癌发生、发展中的作用，应用分子生物学技术，阐明哈族食管癌高发的机理。方法：应用ＰＣＲ技术配合电泳法和ＬＳＡＢ免疫组织化学分析法对新疆地区原发性食管癌及其正常组织中ｐ１６基因第３外显子纯合缺失及基因表达进行检测。结果：４１对标本中癌组织及其正常组织均未发现纯合缺失，２８例标本中１２例有ｐ１６基因表达，占４２．９％（１２／２８），其中哈族为５３．８％（７／１３），汉族为３３．３％（５／１５）。结论：ｐ１６基因第３外显子的纯合缺失在食管癌发病中不是主要因素，ｐ１６基因表达率随食管癌病理分级和临床分期的增加而下降。
Objective: To explore mutation of tumor suppressor gene p16 in Kazak's primary esophageal cancer,and their relationship in the carcino-genesis and progression. Methods: We used PCR combined with PAGE and immunohistochemistry to detect homozygous deletion of p16 exon 3 and expression of pl6 in patients with primary esophageal cancer. Results: No homozygous deletion of p16 exon 3. Expression of pl6 gene in 12 out of 28( 42.9 % ) ES samples were detected, among them in 7 out of 13(53.8 % )Kazak's and in 5 out of 15(33.3 % )Han's esophageal cancer were detected respectively. Conclusion: These results suggest that homozygous deletion of p16 gene exon 3 may not be involved in the pathogenesis and progression of esophageal cancer but there is close relationship between the expression of p16 and tumor grading and clinical staging.
出处
《新疆医科大学学报》
CAS
2000年第1期10-12,共3页
Journal of Xinjiang Medical University
关键词
食管癌
P16抑癌基因
基因缺失
基因表达
esophageal cancer: p16 tumor suppressor gene: immunohistochemistry
homozygous deletion.
