摘要
通过单因素和正交试验确定了超临界CO2萃取啤酒花的萃余物中多酚的最佳提取工艺。结果表明:采用50%vol乙醇为提取溶剂、浸提温度40℃、浸提时间0.5h、超声辅助萃取10min的提取效率最高,在此条件下测定了3啤酒花品种(马可波罗、青岛大花、札一)的萃余物中总多酚的提取率依次为1.909%、1.894%、1.889%。此外,研究了3啤酒花提取物对超氧阴离子的清除活性,结果表明:3种啤酒花萃余物的多酚提取物均具有清除超氧阴离子的活性,其超氧阴离子清除率的IC50值分别为马可波罗6.915mg/mL、青岛大花11.638mg/mL、札一16.509mg/mL。
The extraction conditions of total polyphenols in hop by supercritical CO2 extraction were optimized by using single factor and orthogonal test. The optimal extraction conditions are determined as follows： 50%vol ethanol as extraction solvent, temperature of extract 40℃, 0.5h of extract time and 10min of ultrasonic extraction time. Under these conditions, the yields of abstracting hop polythenols from Marco Polo, Tsingdao flower and SA-1 were 1.909%, 1.894% and 1.889%, respectively. Their activity of IC50 values on scavenging superoxide anion were 6.915mg/ml, 11.638mg/ml, and 16.509mg/ml, respectively.
出处
《中国酿造》
CAS
北大核心
2010年第11期59-62,共4页
China Brewing
基金
新疆维吾尔自治区科技支疆计划项目(200991247)
关键词
超声
啤酒花
总多酚
提取工艺
超氧阴离子
ultrasonic
hop
total polyphenol
extraction technology
superoxide anion
