摘要
通过单因素和正交试验确定了酒花多酚的最佳提取工艺:浸提剂为70%丙酮,料液比1:25(g/ml),回流提取温度45℃,提取时间为1h,在此条件下多酚的提取量达到47.29mg/g干酒花。抗氧化实验结果显示酒花多酚对DPPH自由基的清除能力(IC506.72μg/ml)优于抗坏血酸(IC508.79μg/ml)和单宁酸(IC507.68μg/ml),弱于茶多酚(IC505.77μg/ml);酒花多酚的还原力(EC500.111mg/ml)与抗坏血酸(EC500.101mg/ml)接近,弱于单宁酸(EC500.079mg/ml)和茶多酚(EC500.072mg/ml);酒花多酚对小鼠肝匀浆脂质过氧化产生丙二醛(MDA)的抑制能力(IC500.179mg/ml)优于单宁酸(IC500.462mg/ml),弱于茶多酚(IC500.136mg/ml)。
Confirm the optimum extraction technics of polyphenol of Humulus lupulus by single factor and orthogonal test： impregnant＇ s （acetone） concentration 70%, the ratio of materiel and impregnant 1：25（g/ml）, circumfluence temperature 45℃, time lh and extraction quantity achieve 47.29mg/g dry Humulus lupulus at this condition. The results of anti-oxidative test showed that the polyphenol extracts from Humulus Lupulus has stronger scavenging effects on [DPPH]freedom radical （IC506.72μg/ml） than ascorbic acid （IC50 8.79μg/ml） and tannic acid （IC50 7.68μg/ml）, weaker than tea polyphenols （IC80 5.77μg/ml）; it has nearly reducing capacity （EC50 0.111mg/ml） as ascorbic acid （EC50 0.101mg/ml）, weaker than tannic acid （EC50 0.079mg/ml） and tea polyphenols （EC50 0.072）; it also has stronger inhibition effect on MDA produced by lipid-peroxidation in liver homogenate of small rat （IC50 0.179mg/ml）than tannic acid （IG50 0.462mg/ml）, weaker than tea polyphenols （IC50 0.136mg/ml）.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第10期297-302,共6页
Food Science
基金
山西省自然科学基金资助项目(2006011019)
关键词
酒花多酚
提取工艺
DPPH自由基
还原力
脂质过氧化
polyphenol of Humulus lupulus L
extraction technics
DPPH freedom radical
reducing capacity
lipidperoxidation
