摘要
目的:建立以气相色谱法测定保济丸中百秋李醇含量的方法,制定并完善保济丸的质量标准。方法:采用非极性毛细管柱AT.SE-54(15m×0.25mm×0.33μm),柱温采用程序升温:起始150℃,保持23min,以8℃·min-1速率升至230℃,保持2min,进样口温度为280℃,检测器(FID)温度为280℃,载气为氮气,流速为1.0mL·min-1。结果:百秋李醇进样量在0.12～0.36μg范围内线性关系良好(r=0.9999),平均加样回收率为98.62%,RSD=4.69%。结论:该方法稳定、结果可靠、重复性好,可作为保济丸质量控制标准之一。
OBJECTIVE：To develop gas chromatography （GC） method for the content determination of patchouli alcohol in Baoji pills and to establish the quality standard of it.METHODS：The determination was performed on AT.SE-54 capiltary column （15 m×0.25 mm×0.33 μm）.The increase of column temperature was controlled by programming; the initial temperature was 150 ℃ and maintained for 23 min, and then rose at 8 ℃·min-1 up to 230 ℃ for 2 min.The injector temperature and detector temperature were both 280 ℃.Nitrogen was used as carrier and the flow rate was 1.0 mL·min-1.RESULTS：The linear ranges of patchouli alcohol was 0.12～0.36 μg （r=0.999 9） with an average recovery of 98.62% （RSD=4.69%）.CONCLUSION：The method is accurate, reliable and reproducible, and it can be used for the quality control of Baoji pills.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第40期3833-3834,共2页
China Pharmacy
关键词
保济丸
百秋李醇
气相色谱法
质量标准
Baoji pills
Patchouli alcohol
Gas chromatography
Quality standard
