摘要
采用顶空固相微萃取-气相色谱-质谱联用法(HS-SPME-GC-MS)分离鉴定藿香正气丸中的挥发性成分,通过筛选固相微萃取纤维,优化固相微萃取操作条件,并采用GC-MS对解析物进行分离鉴定.共鉴定出73种组分,主要为烃类倍半萜类化合物.其中,广藿香醇含量最高,为17.6%.结果表明,本实验方法适用于藿香正气丸中挥发性成分的快速分析.
Headspace solid-phase micro-extraction（HS-SPME） technique was employed to extract the volatile compounds from Huoxiang Zhenqi Wan. The volatile components were isolated and identified successfully by gas chromatography-mass spectrometry（GC- MS）. The volatiles were collected using several SPME parameters（including extraction time and temperature）. The best response was obtained using a PDMS ＇fiber by HS- SPME when the extraction temperature was 80 ℃, extraction time was 20 min and desorption time was 5 min. Seventy-three components were identified by HS-SPME. The main components were sesquiterpenoid hydrocarbons compounds. The results showed that the HS-SPME technique can be used to extract the volatile components from HS- SPME-GC-MS.
出处
《汕头大学学报（自然科学版）》
2008年第2期50-55,共6页
Journal of Shantou University：Natural Science Edition
基金
汕头市科技计划资助项目(No:160)
汕头大学青年科研基金资助项目(No:YR07003)
关键词
顶空固相微萃取
气相色谱-质谱联用
挥发性成分
藿香正气丸
headspace solid-phase micro-extraction（HS-SPME）
gas chromatography-mass spectrometry
volatile components
Huoxiang Zhenqi Wan
