摘要
目的：对保济丸中化橘红的分离与含量测定。方法：利用薄层色谱法对保济丸中的化橘红进行定性鉴别。采用Hypersil ODS色谱柱（4.6 mm×250 mm,25 um）,以甲醇-醋酸-水（35∶4∶61）为流动相,检测波长为283 nm,测定柚皮苷含量。结果：供试品薄层色谱图中,在与对照品相应的位置上,显相同颜色的斑点。柚皮苷在0.06-0.054 mg线性关系良好（r=0.999 9）,平均回收率为97.74%,RSD为2.49%。结论：该方法稳定,结果可靠,重复性好,可作为保济丸质量控制标准之一。
Objective： To separate and determine the contents of Naringin in baojiwan pills.Methods： Naringin were detected by thin-layer chromatography.The contents of Naringin in baoji pills was determined by HPLC with Hypersil ODS column,and methanol：acetic acid：water（35：4：61） as a mobile phase,the detection wave length at 283nm.Results： Naringin showed a good linear relationship at a range of 0.06-0.54 mg（r=0.9999）,the average recovery was 97.74%and RSD was2.49%.Conclusion： The method is accurate and reliable,and can be used to control the quality of Baoji pills.
出处
《河南大学学报（医学版）》
CAS
2011年第2期96-98,共3页
Journal of Henan University:Medical Science
