摘要
目的 探索Ⅲ型细胞死亡在重型肝炎中生物学特性和形态学特征及其发病中的意义。 方法用ICM-末端标记(ISEL)、ICM-DNA定量并结合形态学方法,观察17例重型肝炎中细胞死亡。 结果 ①Ⅲ型细胞的ISEL标记阳性,在急性和亚急性重型肝炎中的阳性率为(15±7)个/hpf,高于I型细胞的(5±3)个/hpr(P<0.05),而且无核周空晕,无凋亡小体形成,多分布于大片或亚大片坏死区和坏死边缘区,形态上与Ⅱ型细胞有过渡。②原位DNA定量I型、Ⅲ型细胞中亚2C细胞的百分率分别65％和46％,高于残存肝细胞的11％,(P<0.01～0.05),I型和Ⅱ型细胞中的亚2C分别为65％和29％,也存在差异(P<0.05);I型和Ⅲ型细胞核面积分别为22.09和25.11μm^2,均低于残存肝细胞的59.91μm^2(P<0.05)。③光镜下形态学观察表明:Ⅲ型细胞核染色质边聚,细胞体积缩小等有I型细胞特征,胞浆丰富,胞膜边界不清,又有Ⅱ型细胞特征。结论 Ⅲ型细胞死亡与凋亡和坏死相关,是在重型肝炎坏死区中出现的一种特殊类型的细胞死亡。
Objective To explore the significance of typeⅢ cell death in terms of their biological and morphological characteristics and development in hepatitis gravis. Methods 17 cases of type Ⅲcell death of hepatitis gravis were examined by in situ end labeling (ISEL) and in situ DNA quantitation combined with morphology studies. Results ① In acute and subacute hepatitis,the ISEL positive rate of type Ⅲ cells was 15±7/hpf ,this was higher than the Ⅰ type cells (5±3/hpf) ,P<0. 05. The former cells were without perinuclear halo and apoptotic bodies, and were located mainly in the necrotic or perinecrotic areas,and morphologically had transitional appearance type Ⅱ cells.②In situ DNA quantitation, the positive rates of type Ⅰ ,type Ⅱ and type Ⅲ cells were 65%, 29% and 46% respectively, higher than the residual liver cells (11%),F<0. 01,P<0. 05. The nuclear area of type Ⅰ and type Ⅲ cells 22. 1μm2 and 25-lμm2,were lower than that of the residual liver cells (59. 91μm2) ,P<0. 05. ③Under light microscope,the nuclear chromatin of typeⅢcells being condensed,the cell volume shrank,showing characteristics of type Ⅰ cells,some cells were rich in cytoplasm and had margin showing characteristics of type Ⅱ cells. Conclusion The type Ⅲcell death was related to apoptosis and necrosis,belonging to a special type of cells death,often seen in necrotis areas of hepatitis gravis.
出处
《肝脏》
1999年第2期70-72,共3页
Chinese Hepatology
