摘要
目的探讨Th1、Th2型细胞及Treg细胞在慢性心衰发病中的作用机制。方法采用RT-PCR技术检测62例慢性心衰患者和42例正常人外周血单个核细胞中T-bet、GATA-3及Foxp3mRNA的表达水平;ELISA法检测血浆中Th1/Th2型细胞因子IFN-γ/IL-4水平。结果慢性心衰患者T-bet表达水平高于正常对照组,GATA-3及Foxp3表达水平低于正常对照组(均P<0.05)。慢性心衰组患者血浆中IFN-γ水平及IFN-γ/IL-4高于对照组(均P<0.01)。结论慢性心衰患者T-bet/GATA-3及IFN-γ/IL-4升高,Foxp3降低,提示Th1细胞亚群表达升高,Th2型细胞及CD4+、CD25+调节性T细胞减少,细胞免疫异常可能参与慢性心衰的发病机制。
Objective To explore the roles of Thl, Th2 and Treg cells in pathogenesis of chronic heart failure （CHF）. Methods Real-time PCR was employed to detect the expression of T-bet,GATA-3 and Foxp3 mRNA in pe- ripheral blood mononuclear cells from 62 patients with CHF and 42 healthy controls. ELISA was used to detect serum Th1/Th2 cytokines IFN-γ/IL-4. Results The expression level of T-bet mRNA in the CHF group was higher than that in the control group （P〈0.05）. The expression levels of GATA-3 mRNA and Foxp3 mRNA in the CHF group was lower than that in the control group（P〈0. 05）. The expression levels of IFN-γ/ and IFN-γ/IL-4 in the CHF group was higher than that in the control group（ P〈0. 01）. Conclusion The ratios of T-bet/GATA-3 and IFN-γ/IL-4 increases in patients with CHF, while Foxp3 decreases show that the expression of Th1 cells is higher, and expression of Th2 and Treg is lower. There is a high possibility that the abnormality of cellular immunity may play an important role in the pathogenesis of CHF.
出处
《西部医学》
2014年第3期347-349,共3页
Medical Journal of West China
