摘要
目的：探讨Ｅｎｄｏｓｔａｔｉｎ转基因双歧杆菌口服冻干粉剂（ＥＴＢ－２）对裸鼠人胃癌模型的抑瘤效应，并探讨其抑瘤机制。方法：人胃癌细胞株ＭＫＮ－４５接种于ＢＡＬＢ／Ｌ裸鼠建立动物模型；裸鼠随机分为６组，每组６只，采取灌胃给药。接种后根据肿瘤体积绘制肿瘤生长曲线；给药满４周后处死动物并计算抑瘤率。用免疫组化方法检测微血管密度（ＭＶＤ）和增殖细胞核抗原（ＰＣＮＡ）；用ＤＮＡ原位末端标记（ＴＵＮＥＬ）方法检测细胞凋亡。结果：对照组抑瘤率为０，ＭＶＤ为５．８３１０．８３，细胞凋亡率为１．３０±ｏ．１％；氟铁龙组抑瘤率为４１．１％，ＭＶＤ为５．７０±０．６７，细胞凋亡率为２．７％±０．２％。ＥＴＢ－２高剂量次日给药组抑瘤率为４７．５％，ＭＶＤ为３．９０±０．６７，细胞凋亡率为１３．９％±０．７％，２周给药组抑瘤率为４３．２％，ＭＶＤ为４．２８±０．４９，细胞凋亡率为１３．９％±０．６％；低剂量次日给药组抑瘤率为３６．０％，ＭＶＤ为４．５８±０．３１，细胞凋亡率为７．７％±１．１％。结论：ＥＴＢ－２可显著抑制胃癌生长，其机制可能是抑制肿瘤内微血管形成，使肿瘤细胞增殖下降，诱导肿瘤细胞凋亡，促进肿瘤细胞环死，其作用具有量效?
Background/Aims: To investigate the influence of Endostatin transfected bifidobacterium oral powder preparation (ETB-2) on human gastric cancer and its mechanism of action. Methods: The hu- man gastric cancer cell line MKN-45 was implanted subcutaneously in BALB/L nude mice, then the mice were allocated into 6 groups at random, each group 6 mice. The ETB-2 was given by drenching. The tumor growth curve was got according to the tumor volume, and the rate of tumor inhibition was calculated after sacrificing the animals 4 weeks later. The microvessel density (MVD) and proliferating cell nuclear antigen (PCNA) index were measured immunohistochemically and the apoptotic rate was analyzed with terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) tech- nique. Results: In the controls, the tumor inhibition rate was 0, the MVD was 5.83±0.83, the apopto- tic rate was 1.3%±0.1%, whereas in the fortulon group they were 41.1%, 5.70±0.67 and 2.7%±0.2% respectively. In the high dosage ETB-2 group where ETB-2 was given one day after implantation, the tumor inhibition rate was 47.5%, and MVD 3.90±0.67, the apoptotic rate 13.9%±0.7%; when ETB-2 was given two weeks later, the findings were 43.2%, 4.28±0.49 and 13.9%±0.6% respectively; in the low dosage group, ETB-2 was given one day after implantation, the findings were 36.0%, 4.58±0.31 and 7.7% ±1.1% respectively. Conclusions: ETB-2 can significantly inhibit the development of gastric can- cer, and its action is of dose-dependent, the mechanism of action may be due to its antiangiogenetic effect, inhibiting proliferation, also inducing apoptosis and necrosis.
出处
《胃肠病学》
2001年第3期158-160,191,共4页
Chinese Journal of Gastroenterology
关键词
胃肿瘤
病理性新生血管化
转基因
双歧杆菌
Stomach Neoplasms
Neovascularization, Pathologic
Transgenes
Bifidobacterium
