摘要
目的：观察实验兔急性肺损伤中血管内皮标记物的变化。方法：采用同种骨髓提取液（ＢＭＥ）经兔颈静脉缓慢恒流注入致伤的方法，复制兔骨髓型急性肺损伤（ＡＬＩ）模型，连续６ｈ多时相点观察血中循环内皮细胞（ＣＥＣ）、血浆颗粒膜蛋白－１４０（ＧＭＰ－１４０）和内皮素（ＥＴ－１）以及肺组织ＧＭＰ－１４０含量，并行常规病理及电镜检查。结果：致伤后０．５ｈ，ＣＥＣ、血浆ＧＭＰ－１４０和ＥＴ－１均显著升高，持续６ｈ仍显著高于伤前和对照；其中，血浆ＧＭＰ－１４０升高达３．２５倍，１ｈ时达到高峰，而肺组织ＧＭＰ－１４０含量降低。血浆ＧＭＰ－１４０早期升高，幅度较大，与ＰａＯ２负相关，与其它指标亦有较好的相关性。结论：经兔颈静脉缓慢恒流速注射ＢＭＥ（０．３５ｍｌ／ｋｇ，２ｍｌ／ｈ）能复制出较理想的ＡＬＩ模型。检测血浆ＧＭＰ－１４０可能为临床ＡＬＩ的早期诊断、病情监测和评估提供依据。
Objective: To study the changes of the markers of pulmonary vascular endothelial
cells (PVEC) in acute lung injury (ALI) in rabbits. Methods: 31 rabbits were randomized into the
control group ( n =10) and the bone marrow extract group ( n =21, BME). The animals of BME
group received an injection of 0.35 ml/kg of BME in an injection speed of 2 ml/hour. All rabbits
were killed 6 hours later. Granular membrane protein 140 (GMP 140), endothelin 1 (ET 1) and
circulating endothelial cells (CECs) were measured with immunoradiometric assay (IRMA),
radioimmunoassay (RIA), and immunohistochemistry (IHC) respectively. Results: GMP 140, ET
1 and CEC began to increased in the first 0.5 hour and the increase lasted for 6 hours. The
drammatical elevation of plasma level of GMP 140 (3.25 times) was negatively correlated to
PaO 2 but positively correlated to other parameters. The GMP 140 on the surface of PVECs
was weakly revealed with IHC staining. Conclusion: Slow rate injection of BME can establish
and acceptable model of ALI and the determination of GMP 140 might beaa satisfactory
criterion for early surveillance and for the prognosis of ALI in clinical practice.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1999年第4期241-244,共4页
Journal of Third Military Medical University
基金
全军"九五"重点课题
关键词
肺损伤
肺血管内皮
CEC
GMP-140
内皮素-1
acute lung injury
endothelial cell
pulmonary vasculature
granular membrane protein 140
endothelin 1
rabbit
