摘要
本研究从土样中分离纯化出一株PHB高产菌株P-9,通过对其进行形态学和生理生化反应特征研究,初步鉴定为苏云金芽孢杆菌(Bacillus thuringiensis)。进一步研究P-9菌的生长规律,发现P-9菌生长的延迟期为0-24 h,24 h后进入对数生长期,同时积累PHB,84 h时细胞进入稳定生长期,108 h时PHB产量达到最大,达1.25 g/L。
A strain of P-9 bacteria with high produce of PHB was isolated and purified from soil.P-9 was preliminary appraised as a strain of Bacillus thuringiensis by its characteristics of form and physiology and biochemistry reactions.The lag phase,logarithmic phase and stationary phase was 0-24 h,24-84 h and 84 h,respectively.Further research showed that the maximum yield of PHB in P-9 was 1.25 g/L at 108 h.
出处
《中兽医医药杂志》
2010年第5期40-43,共4页
Journal of Traditional Chinese Veterinary Medicine
基金
铁道部科技研究开发计划No:2010Z001E
甘肃省科技支撑计划(No:D90NKCA079)
国家自然基金(No:3087038)
关键词
聚Β-羟基丁酸
细菌
分离
纯化
poly-β-hydroxybutyrate
bacteria
isolation
purification
