摘要
用野外采回的土样进行菌种分离，得一株编号为Ｂ８的菌株，经初步鉴定该菌株是固氮菌属（ＡｚｏｔｏｂａｃｔｅｒＢｅｉｊｅｒｉｎｃｋ）的一株固氮菌。Ｂ８菌株可利用淀粉在细胞内积累大量的聚β－羟基丁酸（ＰＨＢ），从Ｂ８菌株细胞中提取出的ＰＨＢ，经元素分析、核磁共振及红外光谱检测，结果与Ｓｉｇｍａ产品的性质和其纯度完全一致。经小型发酵试验测定，Ｂ８菌株产ＰＨＢ的量达细胞干重的４１．５％。
By means of analysing the sample collected from field, we have got a kind of strain numbered B8. Prelimilary appraisal may identify it as a branch of Azotobacter Beijerinek. The strain B8 can accumulate a large quantity of poly-β-hydroty butyric acid(PHB) in cells by using starch. The examination of ultimate analysis, nuclear magnetic sonator and infrared spectrum shows that the PHB extracted from bacteria B, has the same nature and purity as the product of sigma. A test of ferment on small scale indicates that the amount of bacteria B8 makes up 41. 5% of the weight of dried cells.
出处
《西北大学学报（自然科学版）》
CAS
CSCD
1996年第2期163-166,共4页
Journal of Northwest University（Natural Science Edition）
基金
陕西省教委专项基金
关键词
聚Β-羟基丁酸
固氮菌
发酵
分离
检测
poly-β-hydroxy butyric acid
Azotobacter Beijerinek
starch
