摘要
目的和方法：实验采用ＰＤＧＦ生物活性检测法，流式细胞术，［３Ｈ］－ＴｄＲ掺入以及细胞免疫组化等方法探讨了低氧状态下，血小板源生长因子（ＰＤＧＦ）及其受体与肺动脉平滑肌细胞（ＰＡＳＭＣ）增殖间的相互关系。结果：低氧可以增强ＰＤＧＦ的活性，上调ＰＤＧＦ受体促进ＰＡＳＭＣ增殖，以ＰＤＧＦ作为刺激剂可进一步促进低氧的促增殖作用。结论：低氧状态下，ＰＡＳＭＣ的ＰＤＧＦ及其受体进一步活化，从而增强了ＰＤＧＦ自分泌、旁分泌作用，由此推测生长因子ＰＤＧＦ是介导低氧致ＰＡＳＭＣ过度增殖的重要发病学环节。
AIM and METHODS:By detecting platelet-derived growth factor(PDGF) activity,immunohistochemistry staining,flow cytometry and -TdR incorporation techniques,we've investigated the relationship between PDGF/its receptors and hypoxic pulmonary artery smooth muscle cells(PASMCs) proliferation.RESULTS:Hypoxia may increase the PDGF activity and upregulate PDGF receptor-α and β.Both hypoxia and PDGF could elevate the number of cells entering S phase from G 0/G 1.CONCLUSIONS:PDGF and its receptors activation allows PASMCs to proliferate to hypoxia.PDGF from hypoxic PASMCs could promote growth in an autocrine and paracrine manner that results in PASMCs proliferative feedback loop and cellular adaptation which may play role in PASMCs over proliferation. \
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1999年第7期598-600,共3页
Chinese Journal of Pathophysiology
基金
国家自然科学基金
关键词
低氧
肺动脉
平滑肌细胞
细胞增殖
PDGF
Anoxia
Pulmonary artery
Muscle,smooth
Cells
Platelet-derived growth factor
