摘要
背景:将生长因子和少量自体骨髓与支架材料复合构成诱导型组织工程化骨,省略了分离及体外培养细胞的步骤和费用,防止了相关的污染和细胞性状改变等,是一种现阶段较为可行的诱导骨组织再生的方法。目的:探讨纳米珍珠层人工骨作为骨形态发生蛋白和自体骨髓载体的可行性,以及其构建的诱导型组织工程化骨治疗股骨头坏死的疗效。方法:健康成年杂种犬建立股骨头坏死模型,分别给予髓芯减压结合植入纳米珍珠层人工骨治疗,单纯髓芯减压治疗。术后4、12周分批处死动物行影像学、生物力学及组织学检查。结果与结论:纳米珍珠层人工骨组的抗压强度高于髓芯减压组和坏死组(P=0.000),纳米珍珠层人工骨组新骨形成明显,表现为软骨内化骨;髓芯减压组未见新骨形成,仅纤维组织填充减压区。结果提示纳米珍珠层人工骨可以作为骨形态发生蛋白和自体骨髓的载体,其构建的诱导型组织工程化骨可改善股骨头抗压强度,促进新骨形成和进行坏死修复。
BACKGROUND：The growth factor compounds a small amount of autologous bone marrow and the scaffold materials to construct the inductive tissue engineered bone,thus omitting steps and costs for isolation and in vitro culture of cells,preventing the related pollution and changes of cell trait,it is a feasible method of inducing bone regeneration.OBJECTIVE：To investigate the feasibility of using nano-nacre artificial bone as a carrier of bone morphogenetic protein and autologous bone marrow,to the curative effect of the constructed inductive tissue engineered bone as a transplant to treat avascular necrosis of femoral head.METHODS：Fifteen healthy hybrid dogs were used to create avascular necrosis of femoral head models.The animal models were treated by core decompression and nano-nacre artificial bone,respectively.The dogs were sacrificed respectively at 4 and 12 weeks postoperation,for radiography,biomechanics and histology examinations.RESULTS AND CONCLUSION：The compressive strength in nano-nacre artificial bone group were significantly superior to those in core decompression group and necrosis group（P=0.000）.New bone formation in nano-nacre artificial bone group was obviously significant and acted as os endochondrale.In core decompression group,the depressor areas were fibrous connective tissue,new bone formation was hardly found.Results demonstrated that it＇s feasible using nano-nacre artificial bone as a carrier of bone morphogenetic protein and autologous bone marrow,the constructed constructed inductive tissue engineered bone can improve compressive strength of femoral head and promote new bone formation and repair necrosis.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第34期6271-6275,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
教育部春晖计划课题基金(22005-2-45001)
广东省科技计划项目(2007B031003005)~~
