摘要
为研发电化学发光体外诊断试剂,设计了一种流动注射池,构建了喷射式流动注射电化学发光分析系统,并运用Ru(bpy)32+/TPA混合进样模式对此系统进行了评估。在检测电位1.18V,进样量100μL,进样速度50μL/s,TPA浓度10mmol/L,样品缓冲液100mmol/LPBS(pH8.4),载体缓冲液10mmol/LPBS(pH8.4)最优条件下,获得Ru(bpy)32+的检出限(S/N=3)为0.005μmol/L;线性范围为0.01～5μmol/L;RSD为1.02%。结果表明,此分析系统灵敏度高,线性范围宽,分析速度快,样品用量少,实现了进样、检测及清洗过程自动化。
Based on the need of developing in vitro diagnostic reagents,a new flow jet detector cell and its analysis system were structured.Ru（bpy） 32 ＋/triprophylamine（TPA） was used to test the capability of this system.Under optimal conditions such as detection potential at 1.18 V,sample injection of 100 μL at 50 μL/s,100 mmol/L of phosphate buffer（pH 8.4） containing 10 mmol/L TPA,running buffer of 10 mmol/L（pH 8.4）,the ECL intensity was linear with the concentrations of Ru（bpy） 23 ＋in the range of 0.01 to 5 μmol/L with a detection limit（S/N = 3） of 0.005 μmol/L and relative standard deviations（RSD） of peak height 1.0%.The results showed that this system had high sensitivity,wide linear range,rapid speed,less sample consumption,and could realize the automation of injection,detection and wash.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2010年第9期1377-1380,共4页
Chinese Journal of Analytical Chemistry
基金
广东省科技计划项目(No2009B030801280)资助
关键词
流动注射
电化学发光
三联吡啶钌
三丙胺
Flow injection
Electrochemiluminescence
Tris（α
α＇-bipyridyl） ruthenium（Ⅱ）
Trispropylamine
