摘要
目的:建立测定人血清帕珠沙星的高效液相色谱的方法,并测定了人血清中帕珠沙星的浓度。方法:色谱条件:LunaC18,粒径5 μm,4.6 mm×150 mm;流动相:乙腈-0.04 mol·L-1磷酸盐缓冲液(1:3,pH=3.1),流速1.0 mL·min～1;柱温28℃;检测波长:242 nm,自动进样器温度为室温,进样量20μL,运行时间为10 min。样品处理采用蛋白沉淀法。结果:线性范围:0.05—40μg·mL～1,最低检测浓度为0.05μg·mL～1,绝对回收率均在95%以上,相对回收率在95%-106%,日内、日间的RSD皆小于15%。结论:适用于临床上测定血清中帕珠沙星的人体药动学的研究。
Objective: To establish a HPLC method for the determination pazufloxacin in human plasma. Methods: HPLC:Use Luna C18column(5 n m,4. 6 mm × 150 mm) the mobile phase consisting of acetonitrile -0.04 mol · L-1 sodium phosphate buffer ( 1: 3,pH =3. 1) ,the flow rate was 1.0 mL · min -0, column temperature 28℃ ,the detection was set at 242 nm. The serum samples were deposited with 15% perchloric acid. Results:The linear range was 0. 05 -40 μg · L-1 and limit of test was 0. 05μg · L-1. Absolute recovery were more than 95% ,relative recovery were 95% -106% ; within - day RSD and inter - day RSD were lass than 15%. Conclusion:This method was found to be sensitive,fast,and accurate. Therefore,it can be used for pharmacokinetic studies of pazufloxacin in human serum.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2004年第5期529-531,共3页
Chinese Journal of Pharmaceutical Analysis
