摘要
目的：建立反相离子对高效液相色谱法以测定尿液中帕珠沙星浓度。方法：色谱条件：Kromasil C18柱（250mm×4．6mm，5μm）；流动相：3mmol／L十二烷基硫酸钠（含0.5％三乙胺，用H3PO4调pH至4．0）-乙腈（62：38，V/V）；流速：1.0mL/min；紫外检测波长：242nm。结果：尿液标准曲线线性范围为1-300mg／L（r=0．9998），高、中、低三种浓度的批内和批间精密度均小于10．0％，提取回收率为94．0％-95．3％。12名健康志愿者分别进行单剂量静脉滴注注射用甲磺酸帕珠沙星后，帕珠沙星在24小时内尿液中的累积尿药排泄率为（90.3±2.6）％。结论：本方法准确可靠，操作简便，适用于临床药代动力学及常规尿药浓度监测。
Objective： To develop a method for the determination of Pazufloxacin concentration in human urine. Methods： Separation was performed on Kromasil C18 capillary column （250 mm× 4.6 mm, 5 μm）. The mobile phase consisted of 3 mmol/L sodium dodecylsulphate （containing 0.5 % triethylamine and adjusted to pH 4.0 with phosphoric acid）-acetonitrile （62：38, V/V）. The flow rate was 1.0 mL/min. The UV detection wavelength was at 242 nm. Results： A good linearity was obtained in the range of 1 - 300 mg/L with r = 0. 9998. The RSD of within-batch and between-batch variation of the method were both less than 10.0 %. The recoveries of three concentrations 1, 30, 300 mg/L were 94.0% - 95.3%. After being given an iv. administration of a single dose of Pazufloxacin rnesilate, the accumulative urine excretion rate of 12 vollanteers during 24 hours was （90.3 ± 2.6）%. Conclusion： This RP-HPLC method is simple, sensitive and accurate. It is suitable for the pharmacokinetic study of Pazufloxacin mesilate and its routine monitoring in human urine.
出处
《药学进展》
CAS
2006年第2期81-83,共3页
Progress in Pharmaceutical Sciences
关键词
帕珠沙星
离子对色谱
累积尿药排泄率
Pazufloxacin
Ion-pair chromatography
Accumulative urine excretion rate
