摘要
利用绿色木霉菌对丹皮提取残渣固态发酵产纤维素酶。以纤维素酶的酶活为指标,丹皮残渣与麸皮的比例、(NH4)2SO4添加量、发酵时间、接种量、发酵温度及固液比为考查因素,采取先单因素后正交实验的方法选取最佳发酵工艺。结果表明,最佳发酵条件为:丹皮残渣与麸皮的比例为2.5∶2.5,(NH4)2SO4添加量3%,发酵时间12天,接种量20%,发酵温度30℃,固液比1∶1.2。丹皮渣固态发酵残渣进行糖化发酵,糖化率为7.6%。因此,丹皮提取残渣固态发酵产纤维素酶是可行的,且发酵后残渣可以直接进行糖化发酵。
It is to research the technology of production of cellulase from TCM residue by Trichoderma viride ZY-1 in solid-state fermentation. The activity of eellulase is taken as the investigative target,through single-factor and orthogonal experiments, to study the effects on the cellulase activity of the ratio of residues of the peony tree root bark and wheat bran, content of （NH4）2SO4,fermentation time,inoculum size,fermentation temperature, and the solid-to-liquid ratio. The results showed that best technical conditions are that the ratio of the residues and wheat bran is 2.5 to 2.5, the content of （NH4）2SO4 is 3%,fermentation time is 12d,inoculum size is 20%,fermentation temperature is 30℃ ,and the solid-toliquid ratio is 1 to 1.2. The fermentation residu6s can be used in the saccharification and the saccharification yield is 7.6%. Therefore,it is viable to use the residues of the peony tree root bark to produce cellulase and the fermentation residues can be saccharified directly.
出处
《安徽化工》
CAS
2010年第B09期57-60,共4页
Anhui Chemical Industry
关键词
中药渣
绿色木霉菌
固态发酵
纤维素酶
TCM residues
Trichoderma viride
solid-state fermentation
cellulase
