摘要
采用固态发酵研究了绿色木霉3.3744菌株产纤维素酶的最佳培养条件.结果表明:最佳碳源为小麦秸粉∶麦麸(4∶6),料∶水=1∶2.5,加入1%(NH4)2SO4,0.1%Tween80,初始pH值为5.0(滤纸酶活)或为3.0(CMC酶活),28℃下培养96 h时其产纤维素酶活力最高.
Trichoderma viride can produce cellulase with high efficiency, the optimal conditions of the solid state fermentation for Trichoderma viride to produce cellulase was studied. The results showed that optimal solid culture medium was that the optimal C source was wheat straw： wheat surface = 4： 6, stuff： water = 1 ： 2.5, adding 1% （NH4）2SO4, 0. 1% Tween80, the optimal original pH is 5.0（for FPA） or 3.0（for CMC）. The optimal culture temperature was 28 ℃, incubation time was 96 hours.
出处
《河南工业大学学报（自然科学版）》
CAS
北大核心
2007年第1期46-48,56,共4页
Journal of Henan University of Technology：Natural Science Edition
基金
河南省优秀青年骨干教师项目(155)
河南工业大学博士启动基金项目
关键词
绿色木霉
纤维素酶
发酵条件
Trichoderma viride
cellulose
culture condition
