摘要
目的旨在观察Ｌ－苯丙氨酸对自发性高血压大鼠心肌成纤维细胞的增殖作用的影响。方法取１天龄自发性高血压大鼠心脏组织培养心肌成纤维细胞，分别以不同浓度的Ｌ－苯丙氨酸、酪氨酸和组氨酸干预一定时间后，利用３Ｈ－胸腺嘧啶掺入技术和细胞计数测定各不同浓度的氨基酸对心肌成纤维细胞ＤＮＡ合成和细胞增殖数目的影响。结果随着Ｌ－苯丙氨酸浓度的增加，心肌成纤维细胞的ＤＮＡ合成量减少，细胞增殖数目减低。而Ｌ－酪氨酸和组氨酸随着浓度的增加对心肌成纤维细胞ＤＮＡ的合成量和细胞增殖数目无影响。结论Ｌ－苯丙氨酸可以特异性地抑制ＳＨＲ心肌成纤维细胞的增殖。
Objective \ To observe the effect of LPhenylalanine on proliferation of
myocardial fibroblast in spontaneosly hypertensive rat.\ Methods \ Amino acids in different
concerntration were added to myocardial fibroblast cultured from 1 dayold spontaneously
hypertensive rat for a defined time. The3Hthymidine incorporation was applied to reflect the
fibroblasts proliferation activity with counts of3Hthymidine per minute divided by the number of
fibroblasts.\ Result\ The DNA synthesis/cell count were decreased with the Phenylalanine in a
dose dependet manner, but not with tyrosine and histidine.\ Conclusion\ LPhenylalanine
inhibited the proliferation of myocardial fibroblast in spontaneously hypertensive rat, which
may explain the proven cases of phenylalanine preventing blood pressure rise and myocardial
hypertrophy in spontaneously hypertensive rat.
出处
《高血压杂志》
CSCD
1999年第2期163-165,共3页
Chinese Journal of Hypertension
基金
国家自然科学基金
关键词
L-苯丙氨酸
高血压
心肌成纤维细胞
LPhenylalanine
\ Spontaneously
hypertensive rat
\ myocardial fibroblast cell
