摘要
研究了深红酵母（Ｒｈｏｄｏｔｏｒｕｌａｒｕｂｒａ）的培养基成分、培养固定化及转化条件。实验表明最佳培养基成分（％）：葡萄糖０５，胰蛋白胨０５，酵母膏０５，磷酸二氢钾００５，ＬＰｈｅ００５，ｐＨ７０，３０℃，２０Ｌ发酵罐中培养１５～１７ｈ。最佳固定化条件为：用２５％卡拉胶包埋１８％的湿菌体。最佳转化条件为：１０％反式肉桂酸，４ｍｏｌ／Ｌ铵离子，ｐＨ１０５，３０℃。
The biotransformation of trans cinnamic acid to L phenylalanine has been studied using phenylalanine ammonia lyase(PAL) containing Rhodotorala rubra .The strains were grown and fully induced for PAL in medium containing:0.5% glucose,0.5% tryptone,0.5% yeast extract,0.05% KH 2PO 4,0.05% L Phe,pH7.0.Cultures were grown at 30℃ in 20L fermenter and harvested after 15～17h growth.For immobilized Rhodotorala rubra cells,maximum conversion degrees were observed using in the trans cinnamic acid concentration of 1.0%,4mol/L NH 4OH.The optimum pH and temperaturefor conversion are 10.5 and 30℃.The best immobilized material and method was that 18% wet cell was immobilized in 2.5% κ carrageenin solution.The κ carrageenin immobilized Rhodotorala rubra cells could convert 77.7% trans cinnamic acid to L phenylanlanine.
出处
《生物工程学报》
CAS
CSCD
北大核心
1997年第1期70-75,共6页
Chinese Journal of Biotechnology
关键词
L-苯丙氨酸
固定化深红酵母
反式肉桂酸
发酵
Rhodotorala rubra ,immotilized,L phenylalanine,trans cinnamic acid κ carrageenin
