摘要
目的:探讨血管紧张素转换酶基因多态性与内蒙古呼和浩特地区汉族人群高血压遗传易感相关性。方法:按WHO标准确诊的原发性高血压患者68例,正常对照67例,取外周血,提取DNA,应用PCR技术和琼脂糖电泳技术进行血管紧张素转换酶基因插入/缺失(I/D)分型,对普通PCR初检为DD型的样本附加插入特异PCR法核对校正。结果:正常对照组中,血管紧张素转换酶基因插入/缺失(I/D)多态DD、DI、II基因型频率分别为0.104、0.508、0.388,其中D和I等位基因频率分别为0.36和0.64;插入/缺失(I/D)多态分布符合Hardy-Weinberg定律,达到遗传平衡,具有群体代表性;高血压组与对照组比较,基因性频率差异无统计学意义(χ2=1.15,P>0.05),等位基因频率之间差异无统计学意义(χ2=0.17,P>0.05)。结论:血管紧张素转换酶基因插入/缺失(I/D)多态性与内蒙古呼和浩特地区汉族人群原发性高血压的发病可能不相关。
Objective：To explore the distribution of I/D polymorphism of ACE gene and its relation to essential hypertension（EH） in inner mongolia Hans.Methods： We studied total of 135 cases,68 cases were diagnosed as EH according to the diagnostic standard of WHO,67 cases were normal contrals;Polymerase Chain Reaction（PCR） and DNA electrophoresis techniques were applied to study ACE gene polymorphism.Results： The frequencies of ACE genotypes DD、DI、II were 0.104、0.508、0.388 in normal contral. The frequencies of D and I in the contral were 0.36 and 0.64.The distribution of I/D polymorphism was in agreement with Hardy-Weinberg equilibrium.The frequencies of ACE genotypes in hypertensives were not significantly different（χ2=1.15,P0.05） from the controls.The frequencies of D and I were not significantly different（χ2=0.17,P0.05） from the controls.Conclusion： From this study we concluded that polymorphism ACE gene I/D is not related to the occurrence of EH in inner mongolia Hans.
出处
《内蒙古医学杂志》
2010年第7期772-774,共3页
Inner Mongolia Medical Journal
关键词
血管紧张素转换酶
原发性高血压
基因多态性
Angiotensin converting enzyme
Essential hypertension
Gene polymorphism
