摘要
目的 探讨血管紧张素转换酶 (ACE)基因I/D多态性与原发性高血压 (EH)及高血压血栓前状态 (PTS)的关系。方法 PCR检测 6 1例原发性高血压病人和正常对照组 2 8例的ACE基因I/D多态性 ;发色底物法测t PA、PAI 1活性 ,酶联免疫吸附双抗夹心法 (ELISA)测vWF含量。结果 高血压组DD基因型频率显著高于对照组 (P <0 0 5 ) ,但D等位基因频率分布在高血压组和正常组之间差异无显著意义 (P >0 0 5 )。高血压组t PA活性降低 ,PAI 1活性、vWF含量升高 (P均 <0 0 0 1)。高血压组DD型t P活性明显低于ID、II型 (P <0 0 0 1) ,而ID、II型之间差异无显著意义 (P >0 0 5 ) ,DD型PAI 1活性明显高于ID、II型(P <0 0 0 1) ,而ID、II型之间差异无显著意义 (P >0 0 5 ) ,vWF在DD、ID、II型三者之间差异无显著意义 (P>0 0 5 )。结论 DD型是原发性高血压发病的危险因素。原发性高血压存在血栓前状态。t PA、PAI 1的变化与血管紧张素转换酶基因I/D多态性有关 ,DD基因型可引起血栓前状态。
Objective To investigate the correlation of insertion/deletion polymorphism of ACE gene with essential hypertension(EH) and prethrombotic state.Methods We detected the ACE I/D polymorphism using PCR technique in 61 essential hypertension patients and 28 control subjects.Plasma t-PA and PAI-1 activities were measured with chromophoric substrate and the concentration of vWF,enzyme linked immunosorbent assay(ELISA).Results The frequency of DD genotype of ACE gene was significantly higher in the essential hypertension groups than that in the control group(P<0.05),but D allele had no difference between the two groups.The markers of endothelium damage and prethrombotic state in the essential hypertension group were higher significantly than those in the control group:there were lower activity of t-PA,higher activity of PAI-1 and higher concentration of vWF in the essential hypertension group than those in the control group(all P<0.001).The activity of t-PA in DD genotype was lower significantly than that in ID or II genotype(P<0.001),but there was no difference between ID and II(P>0.05).The activity of PAI-1 in DD genotype was higher significantly than that in ID or II genotype(P<0.001),but there was no difference between ID and II(P>0.05).The concentration of vWF did not differ between DD,ID and II genotype(all P<0.001).Conclusion DD genotype is a risk factor of essential hypertension.There is a prethrombotic state in essential hypertension.There is a correlation between the variance of t-PA,PAI-1 and ACE I/D polymorphism.DD genotype can cause prethrombotic state.
出处
《中国基层医药》
CAS
2004年第5期521-522,共2页
Chinese Journal of Primary Medicine and Pharmacy
