摘要
为探讨载脂蛋白Ｅ基因多态性与心肌梗死的相关性，以及载脂蛋白Ｅ基因多态性对心肌梗死患者血脂代谢的影响，选取１００例正常人及５０例急性心肌梗死患者，采用聚合酶链反应—限制片长多态性法来分析载脂蛋白Ｅ的基因型，并按常规酶法及免疫法测定血脂和载脂蛋白。结果发现Ｅ３／３基因型及ε３等位基因频率均多见于正常人及病例组中，但两组间Ｅ３／３基因型频率存在明显差异（Ｐ＜０．０５）；ε４等位基因及Ｅ４／３基因型频率在病例组中均明显高于对照组（Ｐ＜０．０１），且病例组中ε４携带者血胆固醇、低密度脂蛋白也均高于Ｅ３／３携带者（Ｐ＜０．０５）。表明载脂蛋白Ｅ基因多态性与急性心肌梗死相关，并影响血胆固醇的代谢。
Aim To investigate the relationship between apolipoprotein E (apo E) genotype and acute myocardial infarction(AMI) and the influence of apolipoprotein E phenotype on serum cholesterol metabolism. Methods Polymorphism of apolipoprotein E gene were detected by polymerase chain reaction fragmant length polymorphism (PCR RFLP) in a cross sectional study of 50 patients with AMI and 100 healthy subjects. The lipids of these subjects were measured with enzyme's methods. Results Comparied with control group, the distribution of apolipoprotein E ε4 allele's and genotype's frequencies in AMI group were increased(P<0.05), patients with ε4 carrier had higher total cholesterol. Low density lipoprotein(LDL) cholesterol and apolipoprotein B concentrations than subjects with homozygous for ε3(P<0.05). Conclusion Apolipoprotein E polymorphism is associated with AMI and affects the metabolism of serum cholesterol.
出处
《中国动脉硬化杂志》
CAS
CSCD
1999年第1期51-53,共3页
Chinese Journal of Arteriosclerosis
关键词
载脂蛋白E
基因型
多态性
心肌梗死
血脂代谢
Apolipoprotein E
Genotype
Polymorphism
Gene Frequency
Myocardial Infarction, Acute
Patients
Cholesterol
