摘要
目的 探讨血管紧张素转换酶 (ACE)基因多态性与老年高血压人群颈动脉粥样硬化的相关性。方法 运用聚合酶链反应 (PCR)技术检测 12 6例老年高血压患者 (高血压组 )及 72例正常老年人 (对照组 )的ACE基因多态性 ,根据PCR检测结果 ,将高血压组分为DD型 (34例 )、Ⅱ型 (4 2例 )及ID型 (5 0例 ) 3个亚组 ,用高分辨超声技术分别检测 3个亚组对象的平均颈动脉内 中膜厚度 (MIMT)、Crouse积分和颈动脉粥样斑块。结果 高血压组ACE基因型及等位基因频率与对照组比较差异无统计学意义 ,3个亚组比较 ,DD型亚组的MIMT、斑块发生率和Crouse积分均较Ⅱ型亚组增高 [(0 .95± 0 .15 )mmvs (0 .85± 0 .11)mm ,82 .35 %vs 5 7.14 % ,均P <0 .0 5和 (7.13± 6 .6 4 )mmvs(2 .6 3± 2 .4 0 )mm ,P <0 .0 1]。结论 ACE基因多态性与老年高血压不相关 ,在高血压人群中 ,ACE基因缺失型具有颈动脉硬化易感性 。
Objective To investigate the relationship between insertion/deletion(I/D) polymorphism of angiotensin converting enzyme(ACE) gene and carotid atherosclerosis in elderly essential hypertension. Methods An I/D polymorphism in ACE gene was detected by polymerase chain reaction(PCR) in 126 cases of elderly essential hypertension(hypertension group) and 72 cases of healthy elders(control group).Based on the I/D polymorphism,the hypertension group was divided into three subgroups: DD type group(n=34),Ⅱ type group(n=42), and ID type group (n=50).The mean intimal-medial thickness (MIMT),Crouse score and plaques of carotid were measured by high-resolution ultrasonography for each of subgroups. Results No significant differences were observed between the hypertension group and control group in frequency of ACE genotype and alleles.Among three subgroups,DD genotype group had higher MIMT,more carotid plaques and higher Crouse score than those in Ⅱ genotype [( 0.95± 0.15) mm vs ( 0.85± 0.11) mm, 82.35% vs 57.14%,all P< 0.05,and ( 7.13± 6.64) mm vs ( 2.63± 2.40) mm,P< 0.01].Conclusion No association was indicated between ACE gene polymorphism and elderly essential hypertension.DD genotype may be a genetic marker responsible for the genetic predisposition to carotid atherosclerosis and vascular damage in essential hypertension.
出处
《临床荟萃》
CAS
北大核心
2004年第12期666-668,共3页
Clinical Focus
基金
深圳市医药卫生科技计划局资助 (2 0 0 30 4 0 2 5)
关键词
血管紧张素转换酶
基因多态性
高血压
动脉硬化
angiotensin-converting enzyme
gene polymorphism
hypertension
atherosclerosis
