摘要
本实验用(60)~Coγ线照射正常人新鲜全血,观察电离辐射对多形核白细胞(PMN)释放超氧化物阴离子自由基(O_2^-)的影响,以及人血甲2巨球蛋白(α_2M)制剂对辐射引起的PMN释放O_2^-的作用。结果表明:正常人新鲜全血照射(5-20Gy)后1h,PMN释放O_2^-的量较不照射组增高(P<0.01),红细胞中超氧化物歧化酶(SOD)的活力较不照射组降低(P<0.01)。照前1h加入人血α_2M制剂(每ml全血中加入138.5单位)能有效地降低PMN的O_2^-释放量,提高红细胞中SOD的活力。离体实验结果提示α_2M治疗辐射损伤作用可能与其抑制过多的O_2^-产生有关。
Peripheral blood of healthy volunteers was irradiated with ^(60)Co in order to study the effects of radiation on superoxide anion radical (?) generation and the inhibitory effects of α_2-macroglobulin (α_2M) blood preparation. It was found that one hour after irradiation, the amount of (?) released from polymorphonuclear leukocytes (PMNs) was more than that of the non-irradiated group (P<0.01) and the activities of superoxide dismutase (SOD) in red cells decreased (P<0.01). When α_2M (138.5 U/ml blood) were added one hour before irradiation, the amount of (?) released from PMNs were reduced and the activities of SOD in red cells were preserved. These results suggest that one reason of α_2M against radiation may be related to inhibition of (?) released from PMNs.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1990年第6期440-443,共4页
Progress In Biochemistry and Biophysics
关键词
早2巨球蛋白
电离辐射
O2^-自由基
polymorphonuclear leukocyte, α_2-macroglobulin, superoxide anion radical
